Chemical Sciences

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Re-examining temporal and seasonal microbial acid mine: drainage community variation
(Laurentian University of Sudbury, 2014-03-19) Auld, Ryan Richard
Acid Mine Drainage (AMD) is characterized by high metal concentrations and an extremely low pH, primarily generated by the microbial oxidation of iron sulfides from mine tailings. Research on the microbial AMD community has largely focused on Bacteria, while little information is known about the Archaeal and Eukaryote members or the seasonal patterns within the communities. Here I examined the Bacterial, Archaeal, and eukaryotic AMD seasonal microbial community, using direct sequencing techniques on AMD samples from the Copper Cliff Tailings AMD site in Sudbury, Ontario, Canada. I found large variation in the community profile and species composition between sampling times of both the Bacterial and Eukaryote communities, suggesting a dynamic community, both between and within seasons. Bacterial diversity was highest during the winter, with Acidithiobacillus dominating, while during the summer, Acidiphilium was the dominant genus. The winter Eukaryote community was dominated by classes of algae and fungi, while the majority of summer sequencing could not be classified to the class level. Few reads were obtained for the Archaeal domain, with low and similar biodiversity between seasons. Overall, the AMD community variation and abundance were found to largely correlate with drainage water and seasonal temperature.