Comparison of analytical tools and biological assays for detecting hydrogen sulfide

Abstract

Hydrogen sulfide (H2S) is a biologically signaling molecule involved in various physiological and pathological processes. The detection and quantification of H2S are critical for understanding its roles in cellular functions and disease mechanisms. This review compares the analytical tools and biological assays used for H2S measurement, focusing on the accuracy, ease of use, and practicality. Analytical tools such as gas chromatography, mass spectrophotometry, and electrochemical sensors offer high precision and sensitivity but often require complex instrumentation and sample preparation. On the other hand, colorimetric, fluorometric, and chemiluminescent assays provide more accessible, real-time detection methods with varying degrees of specificity and interference. Recent advances in probe-based technologies have improved the sensitivity and specificity. These comparisons would help researchers choose the right method for their needs, enhancing the understanding of H2S’s role in health and disease.