Examining the impact of physiologically patterned EMF and LED on the growth of malignant cells: an analysis of the Novel Ca2+ EMF

Abstract

It has been proposed that all living biological systems are capable of emitting or detecting electromagnetic energies as a way of communicating within and between individual units. Should this statement be valid, it opens the door to the possibility of manipulation through artificial application of the appropriately patterned electromagnetic energy. Common forms of electromagnetic energies that are relevant to biological systems include electromagnetic fields and light. Here we explore the possibility for physiologically patterned, frequency modulated electromagnetic fields (EMF) and light-emitting diodes (LED) to influence the growth and viability of malignant (B16-BL6) and non-malignant (HEK 293-T) cell lines. When applied for a single 40- minute exposure, the novel, physiologically patterned Ca2+ EMF reduced the growth of malignant cells 50.3%. Importantly, this same procedure had no effect on non-malignant cells. When applied as an LED, the effects of the Ca2+ pattern were entirely dependent on the wavelength of light. While red (645 nm) LED demonstrated a significant reduction in B16 viable cell counts consistent with the anti-cancer properties of the EMF, blue (470 nm) LED promoted a significant increase in viable cell counts. Finally, we sought to determine the most optimal application parameters of the Ca2+ EMF by adjusting its temporal characteristics. Reducing the point durations from 10 ms to 3 ms further decreased the viability of B16 cells therefore suggesting 3 ms as the optimal time increment for anti-cancer effects. Overall, these results show the ability for specifically patterned EMF and LED to influence the growth of malignant tissues. As such, our findings pose the potential for the Ca2+ EMF to act as a specific, non-invasive, cost- effective therapeutic to be used in the treatment of cancers.